The representation of bullying in Italian primary school children: A mixed-method study comparing drawing and interview data and their association with self-report involvement in bullying events

Bullying continues to be a social issue affecting millions of students of all ages worldwide. Research on bullying seems to be dominated by quantitative research approaches employed standardized categories and measures, ultimately limiting our knowledge about children’s own view on bullying. Our research follows another direction, aiming to explore the representation of bullying in a sample of Italian primary school children by using and comparing the functioning of two qualitative research instruments: interviews, and children’s drawings. In addition, aided by quantitative analyses, we aimed to investigate whether students’ involvement in different bullying roles (as bullies, victims, or defenders), as measured by self-assessment, correlated with different characteristics of the representation of bullying emerging from children’s drawings and interviews. We recruited a convenient sample of 640 primary school students (mean age = 9.44; SD = 0.67), 53.3% of whom were male. The results showed that all forms of bullying, i.e., physical, verbal, and social bullying, could be identified in interview and drawing data, although references to all types of bullying were more frequent in interview data. In terms of bullying criteria, the presence of a power imbalance between the bully and the victim was most frequently detected in both the interview data and the drawing data, while repetition was more easily detected in the interview data. The interview data showed that sadness was the most frequently reported victim emotions, followed by fear, anger, and lack of emotion. The drawing data showed a similar pattern, although victims were more frequently described as lacking emotions compared to the interview data. In both interview and drawing data, age and female gender were positively associated with references to verbal bullying, and negatively associated with references to physical bullying. Additionally, bully/victim children were more likely than uninvolved children to depict physical bullying in the drawings, while this association was not detected in interview data. In summary, our study shows that, compared with drawings, interviews tend to provide a more comprehensive view of children’s own representation of bullying, while drawing data tend to show stronger connections with children’s current personal experiences of bullying

A widespread alternative squalene epoxidase participates in eukaryote steroid biosynthesis

Steroids are essential triterpenoid molecules that are present in all eukaryotes and modulate the fluidity and flexibility of cell membranes. Steroids also serve as signalling molecules that are crucial for growth, development and differentiation of multicellular organisms1-3. The steroid biosynthetic pathway is highly conserved and is key in eukaryote evolution4-7. The flavoprotein squalene epoxidase (SQE) catalyses the first oxygenation reaction in this pathway and is rate limiting. However, despite its conservation in animals, plants and fungi, several phylogenetically widely distributed eukaryote genomes lack an SQE-encoding gene7,8. Here, we discovered and characterized an alternative SQE (AltSQE) belonging to the fatty acid hydroxylase superfamily. AltSQE was identified through screening of a gene library of the diatom Phaeodactylum tricornutum in a SQE-deficient yeast. In accordance with its divergent protein structure and need for cofactors, we found that AltSQE is insensitive to the conventional SQE inhibitor terbinafine. AltSQE is present in many eukaryotic lineages but is mutually exclusive with SQE and shows a patchy distribution within monophyletic clades. Our discovery provides an alternative element for the conserved steroid biosynthesis pathway, raises questions about eukaryote metabolic evolution and opens routes to develop selective SQE inhibitors to control hazardous organisms

The Interreg IV Italia-Austria "SeismoSAT" Project: connecting Seismic Data Centers via satellite

Since 2002 the Istituto Nazionale di Oceanografia e di Geofisica Sperimentale - OGS in Udine (Italy), the Zentralanstalt für Meteorologie und Geodynamik (ZAMG) in Vienna (Austria), and the Agencija Republike Slovenije za okolje (ARSO) in Ljubljana (Slovenija) are using the Antelope software suite as the main tool for collecting, analyzing, archiving and exchanging seismic data in real time, initially in the framework of the EU Interreg IIIA project “Trans-national seismological networks in the South-Eastern Alps”. The data exchange has proved to be effective and very useful in case of seismic events near the borders between Italy, Austria and Slovenia, where the poor single national seismic networks coverage precluded a correct localization, while the usage of common data from the integrated networks improves considerably the overall reliability of real time seismic monitoring of the area. At the moment the data exchange between the seismic data centers relies on internet: this however is not an ideal condition for civil protection purposes, since internet reliability is poor. For this reason in 2012 the Protezione Civile della Provincia Autonoma di Bolzano in Bolzano (Italy) joined OGS, ZAMG and ARSO in the Interreg IV Italia-Austria Project “SeismoSAT” aimed in connecting the seismic data centers in real time via satellite. The general schema of the project, including first data bandwith estimates and a possible architecture will be illustrated

Behind the Mirror: Chirality Tunes the Reactivity and Cytotoxicity of Chloropiperidines as Potential Anticancer Agents

The pressing demand for sustainable antitumor drugs prompted us to investigate 3-chloropiperidines as potential mustard-based anticancer agents. In this study, an explorative set of variously decorated monofunctional 3-chloropiperidines (M-CePs) was efficiently synthesized through a fast and affordable route providing high yields of pure racemates and enantiomers. Consistently with their reactivity, M-CePs were demonstrated to alkylate DNA in vitro. On a panel of carcinoma cell lines, M-CePs exhibited low nanomolar cytotoxicity indexes, which showed their remarkable activity against pancreatic cancer cells and in all cases performed strikingly better than the chlorambucil control. Very interestingly, stereochemistry modulated the activity of M-CePs in unexpected ways, pointing to additional molecular mechanisms of action beyond the direct damage of genomic DNA. This encouraging combination of efficacy and sustainability suggests they are valid candidates for anticancer agent development

Detailed proteomic analysis on DM: insight into its hypoallergenicity.

Successful therapy in cow milk (CM) protein allergy rests upon completely eliminating CM proteins from the child's diet: it is thus necessary to provide a replacement food. Donkey milk (DM) has recently aroused scientific and clinical interest, above all among paediatric allergologists. A deeper knowledge of proteins in DM is necessary to evaluate the immunological and physiological properties of this natural substitute for cow's milk. The paper offers a detailed comparative analysis among the protein fractions of DM, CM and human milk, following an extensive proteomic study of the casein and whey proteins of DM performed by narrow pH range 2-DE. The detailed protein composition and structural features reported in this study provide insight into the molecular reasons for the hypoallergenicity of DM. Whole DM might constitute a valid substitute of CM in feeding children with CM protein allergy and it might also constitute the basis for formulas suitable for allergic subjects in the first year of life